Oncogene HGNC: 3236

EGFR

Epidermal Growth Factor Receptor

OncoKB ↗ Civic ↗ Ensembl ↗

cCCLE Frequency

18.4% ↑ 2.1% higher than TCGA

Hotspot Variant

L858R Exon 21 Substitution

FDA Approved Drugs

Osimertinib Gefitinib

Locus

Chr7: 55,019,017 - 55,211,628 GRCh38/hg38

Summary: EGFR is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. Overexpression or mutations in this gene are associated with various cancers, particularly Lung Adenocarcinoma and Glioblastoma. In the cCCLE cohort, it shows a distinctive hotspot enrichment in East Asian NSCLC models.

Select Model Subset

Cohort Definition

Data Source

Primary Lineage

Sub-lineage

580 Models Selected

Mutation Profile 🧬

18.4%

Hotspot: L858R

Expression 📊

42.8 TPM

Highest: Liver models

Copy Number ✂️

24 Models

Status: Deep Amplification

Amp Del

Fusion/SV 🔗

5 Events

Frequent: EGFR-SEPT14

2 VALIDATED BY WGS

Mutation Landscape (EGFR)

RefSeq: NM_005228 | 1,210 Amino Acids

cCCLE Freq

18.4%

Total Models

108 / 580

A. Somatic Mutation Hotspots

Missense Truncating

Receptor_L

Furin-like

GF_recep_IV

Pkinase_Tyr

E709K

Exon19_Del

T790M

L858R

L861Q

B. Cohort Waterfall (Mutated Models)

Ranked by Variant Type

Mutated (N=108) Wild-Type (N=472)

Hotspots In-frame Del Others

Models Carrying EGFR Variants

Model Name	Source	Amino Acid Change	Variant Type	VAF	Link
PC-9	cCCLE	p.E746_A750del	In-frame Deletion	0.82	Profile →
H1975	ATCC	p.L858R, p.T790M	Missense (Double)	0.45, 0.42	Profile →
HCC827	cCCLE	p.E746_A750del	In-frame Deletion	0.91	Profile →

Expression vs. Copy Number

Correlation across multiple RNA biotypes

mRNA Expression Correlation

R = 0.78

N=582 Models

Copy Number (log2 Ratio)

mRNA Expression (log2 TPM)

Model Name	Lineage	mRNA (TPM)	Copy Number (log2)	Action
Hep-G2	Liver	12.84	+1.92	Profile →

Fusion & Structural Variation

Large-scale Genomic Rearrangements

Detected in 12 Models

Structural Variant Event List

Model	Event Type	Breakpoints (5' ⟷ 3')	Frame Status	Details
U-87 MG	Fusion	EGFR(e24) ⟷ SEPTIN14(e10)	In-Frame	cCCLE Internal
A549	Deletion (SV)	Chr7:55,120,442 ⟷ 55,142,301	—	Exon 2-7 Loss